{"id":1851,"date":"2022-02-07T18:46:00","date_gmt":"2022-02-07T17:46:00","guid":{"rendered":"https:\/\/glycanostics.com\/?p=1851"},"modified":"2022-07-08T08:42:04","modified_gmt":"2022-07-08T06:42:04","slug":"pokrocily-konfiguracny-a-analyticky-protokol-pre-impedimetricke-biosenzory-na-glykoprofilaciu-prostatickeho-onkomarkera-pomocou-zlatych-nanocastic-s-magnetickym-jadrom","status":"publish","type":"post","link":"https:\/\/glycanostics.com\/sk\/pokrocily-konfiguracny-a-analyticky-protokol-pre-impedimetricke-biosenzory-na-glykoprofilaciu-prostatickeho-onkomarkera-pomocou-zlatych-nanocastic-s-magnetickym-jadrom\/","title":{"rendered":"Pokro\u010dil\u00fd konfigura\u010dn\u00fd a analytick\u00fd protokol pre impedimetrick\u00e9 biosenzory na glykoprofil\u00e1ciu prostatick\u00e9ho onkomarkera pomocou zlat\u00fdch nano\u010dast\u00edc s magnetick\u00fdm jadrom"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"1851\" class=\"elementor elementor-1851\">\n\t\t\t\t\t\t\t\t\t<section data-particle_enable=\"false\" data-particle-mobile-disabled=\"false\" class=\"elementor-section elementor-top-section elementor-element elementor-element-3d54ab08 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"3d54ab08\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-5f4ff1da\" data-id=\"5f4ff1da\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-672f454 elementor-widget elementor-widget-heading\" data-id=\"672f454\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">Pokro\u010dil\u00fd konfigura\u010dn\u00fd a analytick\u00fd protokol pre impedimetrick\u00e9 biosenzory na glykoprofil\u00e1ciu prostatick\u00e9ho onkomarkera pomocou zlat\u00fdch nano\u010dast\u00edc s magnetick\u00fdm jadrom<\/h1>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-73c3bfbc elementor-widget elementor-widget-text-editor\" data-id=\"73c3bfbc\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>In this paper several advances were implemented for glycoprofiling of prostate specific antigen (PSA), what can be applied for better prostate cancer (PCa) diagnostics in the future: 1) application of Au nanoshells with a magnetic core (MP@silica@Au); 2) use of surface plasmons of Au nanoshells with a magnetic core for spontaneous immobilization of zwitterionic molecules via diazonium salt grafting; 3) a double anti-fouling strategy with integration of zwitterionic molecules on Au surface and on MP@silica@Au particles was implemented to resist non-specific protein binding; 4) application of anti-PSA antibody modified Au nanoshells with a magnetic core for enrichment of PSA from a complex matrix of a human serum; 5) direct incubation of anti-PSA modified MP@silica@Au with affinity bound PSA to the lectin modified electrode surface. The electrochemical impedance spectroscopy (EIS) signal was enhanced 43 times integrating Au nanoshells with a magnetic core compared to the biosensor without them. This proof-of-concept study shows that the biosensor could detect PSA down to 1.2 fM and at the same time to glycoprofile such low PSA concentration using a lectin patterned biosensor device. The biosensor offers a recovery index of 108%, when serum sample was spiked with a physiological concentration of PSA (3.5 ng mL\u22121).<\/p><p><a href=\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S0956566319300818?via%3Dihub\" target=\"_blank\" rel=\"noopener\">More info&#8230;<\/a><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>In this paper several advances were implemented for glycoprofiling of prostate specific antigen (PSA), what can be applied for better prostate cancer (PCa) diagnostics in the future: 1) application of Au nanoshells with a magnetic core (MP@silica@Au); 2) use of surface plasmons of Au nanoshells with a magnetic core for spontaneous immobilization of zwitterionic molecules [&hellip;]<\/p>\n","protected":false},"author":4,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"elementor_header_footer","format":"standard","meta":{"inline_featured_image":false,"_mi_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[25],"tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v21.5 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Pokro\u010dil\u00fd konfigura\u010dn\u00fd a analytick\u00fd protokol pre impedimetrick\u00e9 biosenzory na glykoprofil\u00e1ciu prostatick\u00e9ho onkomarkera pomocou zlat\u00fdch nano\u010dast\u00edc s magnetick\u00fdm jadrom - Glycanostics<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S0956566319300818\/?via=ihub\" \/>\n<meta property=\"og:locale\" content=\"sk_SK\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Pokro\u010dil\u00fd konfigura\u010dn\u00fd a analytick\u00fd protokol pre impedimetrick\u00e9 biosenzory na glykoprofil\u00e1ciu prostatick\u00e9ho onkomarkera pomocou zlat\u00fdch nano\u010dast\u00edc s magnetick\u00fdm jadrom - Glycanostics\" \/>\n<meta property=\"og:description\" content=\"In this paper several advances were implemented for glycoprofiling of prostate specific antigen (PSA), what can be applied for better prostate cancer (PCa) diagnostics in the future: 1) application of Au nanoshells with a magnetic core (MP@silica@Au); 2) use of surface plasmons of Au nanoshells with a magnetic core for spontaneous immobilization of zwitterionic molecules [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S0956566319300818\/?via=ihub\" \/>\n<meta property=\"og:site_name\" content=\"Glycanostics\" \/>\n<meta property=\"article:published_time\" content=\"2022-02-07T17:46:00+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2022-07-08T06:42:04+00:00\" \/>\n<meta name=\"author\" content=\"cagan@seesame.com\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Autor\" \/>\n\t<meta name=\"twitter:data1\" content=\"cagan@seesame.com\" \/>\n\t<meta name=\"twitter:label2\" content=\"Predpokladan\u00fd \u010das \u010d\u00edtania\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 min\u00fata\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S0956566319300818\/?via%3Dihub\",\"url\":\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S0956566319300818\/?via%3Dihub\",\"name\":\"Pokro\u010dil\u00fd konfigura\u010dn\u00fd a analytick\u00fd protokol pre impedimetrick\u00e9 biosenzory na glykoprofil\u00e1ciu prostatick\u00e9ho onkomarkera pomocou zlat\u00fdch nano\u010dast\u00edc s magnetick\u00fdm jadrom - Glycanostics\",\"isPartOf\":{\"@id\":\"https:\/\/glycanostics.com\/#website\"},\"datePublished\":\"2022-02-07T17:46:00+00:00\",\"dateModified\":\"2022-07-08T06:42:04+00:00\",\"author\":{\"@id\":\"https:\/\/glycanostics.com\/#\/schema\/person\/badee4cbfc54e0bf99314635b428c54d\"},\"breadcrumb\":{\"@id\":\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S0956566319300818\/?via%3Dihub#breadcrumb\"},\"inLanguage\":\"sk-SK\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S0956566319300818\/?via%3Dihub\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S0956566319300818\/?via%3Dihub#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/glycanostics.com\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Pokro\u010dil\u00fd konfigura\u010dn\u00fd a analytick\u00fd protokol pre impedimetrick\u00e9 biosenzory na glykoprofil\u00e1ciu prostatick\u00e9ho onkomarkera pomocou zlat\u00fdch nano\u010dast\u00edc s magnetick\u00fdm jadrom\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/glycanostics.com\/#website\",\"url\":\"https:\/\/glycanostics.com\/\",\"name\":\"Glycanostics\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/glycanostics.com\/?s={search_term_string}\"},\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"sk-SK\"},{\"@type\":\"Person\",\"@id\":\"https:\/\/glycanostics.com\/#\/schema\/person\/badee4cbfc54e0bf99314635b428c54d\",\"name\":\"cagan@seesame.com\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"sk-SK\",\"@id\":\"https:\/\/glycanostics.com\/#\/schema\/person\/image\/\",\"url\":\"https:\/\/secure.gravatar.com\/avatar\/7849fc5f0186381f2497fdb687e3b6dd?s=96&d=mm&r=g\",\"contentUrl\":\"https:\/\/secure.gravatar.com\/avatar\/7849fc5f0186381f2497fdb687e3b6dd?s=96&d=mm&r=g\",\"caption\":\"cagan@seesame.com\"},\"url\":\"https:\/\/glycanostics.com\/sk\/author\/caganseesame-com\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Pokro\u010dil\u00fd konfigura\u010dn\u00fd a analytick\u00fd protokol pre impedimetrick\u00e9 biosenzory na glykoprofil\u00e1ciu prostatick\u00e9ho onkomarkera pomocou zlat\u00fdch nano\u010dast\u00edc s magnetick\u00fdm jadrom - Glycanostics","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S0956566319300818\/?via=ihub","og_locale":"sk_SK","og_type":"article","og_title":"Pokro\u010dil\u00fd konfigura\u010dn\u00fd a analytick\u00fd protokol pre impedimetrick\u00e9 biosenzory na glykoprofil\u00e1ciu prostatick\u00e9ho onkomarkera pomocou zlat\u00fdch nano\u010dast\u00edc s magnetick\u00fdm jadrom - Glycanostics","og_description":"In this paper several advances were implemented for glycoprofiling of prostate specific antigen (PSA), what can be applied for better prostate cancer (PCa) diagnostics in the future: 1) application of Au nanoshells with a magnetic core (MP@silica@Au); 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